Influence of growth regulators (2, 4-D and Kinetin) on vegetative and reproductive behaviour of some green algae

A detailed study was done to understand the effect of phytohormones on morphology and growth performances of green filamentous algal taxa viz. Pithophora mooreana, P. cleveana, P. roettleri, Cladophora glomerata, Spirogyra orientalis, Oedogonium hindustanense, O. mexicanum, O. lemmermannii, O. rufescens f. minuta and Trentepohlia torulosa in vitro. Different proportion of 2, 4-Dichlorophenoxy acetic acid (2, 4-D) and kinetin (2:1 and 1:2 of 1mg/mL concentration) were applied in growth medium in laboratory condition. Drastic change in cell proliferation, cell enlargement and initiation of reproductive structures were observed. Both cell length and breadth were increased with time at high 2, 4-D and low kinetin ratio (2:1). The statistical analysis showed significant relationship of cell length and breadth under the variables 2:1 and 1:2 of P. roettleri (p<0.05). A significant result (p<0.05) was obtained in cell length of O. lemmermannii and cell breadth in P. mooreana under two variables, 2:1 and 1:2 hormone ratios. High 2, 4-D level induced formation of reproductive structures viz. sporangium, oogonium, akinetes etc whereas its low concentration does not show any major changes. The high kinetin induced rapid cell division which results cell proliferation and enlargement. The detail morphological and reproductive changes are reported in the present communication.